02-09-2017, 03:38
In separate studies the conclusion was the same (lower dose astaxanthin did not reduce estradiol). In the first study Astaxanthin @ 2000mg inhibited DHT and lowered estradiol....(btw, who takes 2000 mg(s) of astaxanthin anyways?) and 800 mg of astaxathin did not reduce E2.
That's the important (and often overlooked) part of that information. It's Saw Palmetto reducing estradiol....not Astaxanthin....why???, the study indicates the result is dose dependent, meaning the 2000 mg dose of the SP/astaxanthin supplement (they studied) overshadows the astaxanthin. My calculations showed that only .3 mg of astaxanthin inhibits DHT at an astonishing 98%.
So right there, the inhibition of DHT at 98% tells us exactly what?.....it leaves E2 to exert domination.....(aka- a pro-breast growth scenario). Look at what astaxanthin does in addition to just inhibiting DHT in this PDF below.
Astaxanthin and Type 2 Diabetes
New Evidences Emerging from Human Studies
In an anti-aging study conducted by Iwabayashi et al., (2009), 20 female volunteers with increased oxidative stress burden ingested 12 mg/day of astaxanthin for 8 weeks. Results evidenced a significant decrease of diabetes-related parameters that collectively predict trends in diabetes development. Firstly, astaxanthin reduced cortisol by 23 percent (p<0.05). High levels of cortisol decreases metabolism of glucose, which contributes to increased blood glucose and fat levels that eventually lead to insulin resistance. Secondly, astaxanthin reduced LDH by 6.5% percent(p<0.01). Overexpression of LDH activity interferes with normal glucose metabolism and insulin secretion. Thirdly, astaxanthin decreased the glycated hemoglobin molecules HbA1c by 4% (p<0.01) a direct indication of the level of glucose in the blood.
https://pugos.in/wp-content/uploads/2016...0.2010.pdf
I'll repost this information from last year (again).
J Int Soc Sports Nutr. 2008 Aug 12;5:12. doi: 10.1186/1550-2783-5-12.
An open label, dose response study to determine the effect of a dietary supplement on dihydrotestosterone, testosterone and estradiol levels in healthy males. https://www.ncbi.nlm.nih.gov/pubmed/18700016
In a new find, Astaxanthin promotes P4 (progesterone)...
Astaxanthin increases progesterone production in cultured bovine luteal cells
Hachiro KAMADA,1,* Satoshi AKAGI,1 and Shinya WATANABE1
Abstract
Although astaxanthin (AST) is known to be a strong antioxidant, its effects on reproductive function in domestic animals have not yet been elucidated in detail. Therefore, we investigated the effects of AST on luteal cells, which produce progesterone (P4), an important hormone for maintaining pregnancy. Luteal cells were prepared by collagenase dispersion of the corpus luteum (CL). The addition of racemic AST at a low concentration (<10 nM) to cultured bovine luteal cells increased P4 in the culture medium (P<0.05). This effect was attributed to an increase in the ability of luteal cells to produce P4 (P4/cell·DNA); however, the level of lipid peroxide (TBARS: thiobarbituric acid reactive substances) per cell did not decrease with the addition of AST, whose values were similar to that with the addition of luteinizing hormone. When optical isomers of AST (SS and RR types) were added to the culture medium, respectively, SS-AST was more effective in increasing P4 production than RR-AST. When 1 mg/kg·body weight of SS-AST derived from green algae was fed to cows for 2 weeks, its concentration in blood plasma was 10.9 nM on average, which was sufficient to expect an in vitro effect on the production of P4 in cows. These results suggested the potential of SS-AST supplements for cows to elevate luteal function.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5487791/
Astaxanthin Inhibits PC-3 Xenograft Prostate Tumor Growth in Nude Mice
Xiaofeng Ni,1 Haining Yu,2 Shanshan Wang,1 Chengcheng Zhang,1 and Shengrong Shen1,* Keith B. Glaser, Academic Editor
Abstract
Prostate cancer (PCa), the most common malignancy in men, is a major cause of cancer deaths. A better understanding of the mechanisms that drive tumor initiation and progression may identify actionable targets to improve treatment of this patient group. As a dietary carotenoid, astaxanthin has been demonstrated to exert beneficial effects against inflammation, cardiovascular disease, oxidative damage, or different cancer sites. This study used intragastric administration of astaxanthin to detect its role on tumor proliferation, apoptosis, microRNA (miRNA) overexpression, and microbacteria composition change by establishing androgen-independent PCa cell PC-3 xenograft nude mice. Nude mice were inoculated with androgen-independent prostate cancer PC-3 cells subcutaneously. The intervention was started when tumors reached 0.5–0.6 cm in diameter. Mice were intragastrically administered 100 mg/kg astaxanthin (HA), 25 mg/kg astaxanthin (LA), or olive oil (TC). The results showed that 100 mg/kg astaxanthin significantly inhibited tumor growth compared to the TC group, with an inhibitory rate of 41.7%. A decrease of Ki67 and proliferating cell nuclear antigen (PCNA) as well as an increase of cleaved caspase-3 were observed in HA-treated tumors, along with increasing apoptotic cells, obtained by TUNEL assay. The HA significantly elevated the levels of tumor suppressors miR-375 and miR-487b in tumor tissues and the amount of Lactobacillus sp. and Lachnospiraceae in mice stools, while there was no significant difference between LA and TC groups. These results provide a promising regimen to enhance the therapeutic effect in a dietary supplement manner.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5367023/
J Herb Pharmacother. 2005;5(1):17-26.
A preliminary investigation of the enzymatic inhibition of 5alpha-reduction and growth of prostatic carcinoma cell line LNCap-FGC by natural astaxanthin and Saw Palmetto lipid extract in vitro.
Anderson ML1.
Abstract
Inhibition of 5alpha-reductase has been reported to decrease the symptoms of benign prostate hyperplasia (BPH) and possibly inhibit or help treat prostate cancer. Saw Palmetto berry lipid extract (SPLE) is reported to inhibit 5alpha-reductase and decrease the clinical symptoms of BPH. Epidemiologic studies report that carotenoids such as lycopene may inhibit prostate cancer. In this investigation the effect of the carotenoid astaxanthin, and SPLE were examined for their effect on 5alpha-reductase inhibition as well as the growth of prostatic carcinoma cells in vitro. These studies support patent #6,277,417 B1. The results show astaxanthin demonstrated 98% inhibition of 5alpha-reductase at 300 microg/mL in vitro. Alphastat, the combination of astaxanthin and SPLE, showed a 20% greater inhibition of 5alpha-reductase than SPLE alone n vitro. A nine day treatment of prostatic carcinoma cells with astaxanthin in vitro produced a 24% decrease in growth at 0.1 mcg/mL and a 38% decrease at 0.01 mcg/mL. SPLE showed a 34% decrease at 0.1 mcg/mL.
CONCLUSIONS:
Low levels of carotenoid astaxanthin inhibit 5alpha-reductase and decrease the growth of human prostatic cancer cells in vitro. Astaxanthin added to SPLE shows greater inhibition of 5alpha-reductase than SPLE alone in vitro.
Quote:Blood levels of ES (estrogen sulfate) however, decreased significantly (p = 0.05) in the 2000 mg/day dose group but not in the 800 mg/day dose group indicating a dose dependant decrease in E levels.
That's the important (and often overlooked) part of that information. It's Saw Palmetto reducing estradiol....not Astaxanthin....why???, the study indicates the result is dose dependent, meaning the 2000 mg dose of the SP/astaxanthin supplement (they studied) overshadows the astaxanthin. My calculations showed that only .3 mg of astaxanthin inhibits DHT at an astonishing 98%.
So right there, the inhibition of DHT at 98% tells us exactly what?.....it leaves E2 to exert domination.....(aka- a pro-breast growth scenario). Look at what astaxanthin does in addition to just inhibiting DHT in this PDF below.
Astaxanthin and Type 2 Diabetes
New Evidences Emerging from Human Studies
In an anti-aging study conducted by Iwabayashi et al., (2009), 20 female volunteers with increased oxidative stress burden ingested 12 mg/day of astaxanthin for 8 weeks. Results evidenced a significant decrease of diabetes-related parameters that collectively predict trends in diabetes development. Firstly, astaxanthin reduced cortisol by 23 percent (p<0.05). High levels of cortisol decreases metabolism of glucose, which contributes to increased blood glucose and fat levels that eventually lead to insulin resistance. Secondly, astaxanthin reduced LDH by 6.5% percent(p<0.01). Overexpression of LDH activity interferes with normal glucose metabolism and insulin secretion. Thirdly, astaxanthin decreased the glycated hemoglobin molecules HbA1c by 4% (p<0.01) a direct indication of the level of glucose in the blood.
https://pugos.in/wp-content/uploads/2016...0.2010.pdf
I'll repost this information from last year (again).
(22-09-2016, 21:37)Lotus Wrote: Astaxanthin inhibits DHT, free radicals, cancer, radiation, diabetes and protects DNA.. Maybe 8-12 mg is too much, the study demostrated (in vitro) .3 mg was to inhibit DHT @ 99% (type II most likely). I found in further studies that astaxanthin inhibits DHT while promoting T. In other words, an ideal scenario for pro-aromatase (increased estrogen), though incombination with a pro-aromatase. So, maybe 2mg -4mg would be a good starting dose.
(06-02-2016, 05:29)Lotus Wrote: A preliminary investigation of the enzymatic inhibition of 5alpha-reduction and growth of prostatic carcinoma cell line LNCap-FGC by natural astaxanthin and Saw Palmetto lipid extract in vitro.
J Herb Pharmacother. 2005;5(1):17-26.
Abstract
Inhibition of 5alpha-reductase has been reported to decrease the symptoms of benign prostate hyperplasia (BPH) and possibly inhibit or help treat prostate cancer. Saw Palmetto berry lipid extract (SPLE) is reported to inhibit 5alpha-reductase and decrease the clinical symptoms of BPH. Epidemiologic studies report that carotenoids such as lycopene may inhibit prostate cancer. In this investigation the effect of the carotenoid astaxanthin, and SPLE were examined for their effect on 5alpha-reductase inhibition as well as the growth of prostatic carcinoma cells in vitro. These studies support patent #6,277,417 B1. The results show astaxanthin demonstrated 98% inhibition of 5alpha-reductase at 300 microg/mL in vitro. Alphastat, the combination of astaxanthin and SPLE, showed a 20% greater inhibition of 5alpha-reductase than SPLE alone n vitro. A nine day treatment of prostatic carcinoma cells with astaxanthin in vitro produced a 24% decrease in growth at 0.1 mcg/mL and a 38% decrease at 0.01 mcg/mL. SPLE showed a 34% decrease at 0.1 mcg/mL.
CONCLUSIONS: Low levels of carotenoid astaxanthin inhibit 5alpha-reductase and decrease the growth of human prostatic cancer cells in vitro. Astaxanthin added to SPLE shows greater inhibition of 5alpha-reductase than SPLE alone in vitro.
PMID 16093232 [PubMed - indexed for MEDLINE]
Anti-cancer potential of flavonoids: recent trends and future perspectives
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3824783/
J Int Soc Sports Nutr. 2008 Aug 12;5:12. doi: 10.1186/1550-2783-5-12.
An open label, dose response study to determine the effect of a dietary supplement on dihydrotestosterone, testosterone and estradiol levels in healthy males. https://www.ncbi.nlm.nih.gov/pubmed/18700016
In a new find, Astaxanthin promotes P4 (progesterone)...
Astaxanthin increases progesterone production in cultured bovine luteal cells
Hachiro KAMADA,1,* Satoshi AKAGI,1 and Shinya WATANABE1
Abstract
Although astaxanthin (AST) is known to be a strong antioxidant, its effects on reproductive function in domestic animals have not yet been elucidated in detail. Therefore, we investigated the effects of AST on luteal cells, which produce progesterone (P4), an important hormone for maintaining pregnancy. Luteal cells were prepared by collagenase dispersion of the corpus luteum (CL). The addition of racemic AST at a low concentration (<10 nM) to cultured bovine luteal cells increased P4 in the culture medium (P<0.05). This effect was attributed to an increase in the ability of luteal cells to produce P4 (P4/cell·DNA); however, the level of lipid peroxide (TBARS: thiobarbituric acid reactive substances) per cell did not decrease with the addition of AST, whose values were similar to that with the addition of luteinizing hormone. When optical isomers of AST (SS and RR types) were added to the culture medium, respectively, SS-AST was more effective in increasing P4 production than RR-AST. When 1 mg/kg·body weight of SS-AST derived from green algae was fed to cows for 2 weeks, its concentration in blood plasma was 10.9 nM on average, which was sufficient to expect an in vitro effect on the production of P4 in cows. These results suggested the potential of SS-AST supplements for cows to elevate luteal function.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5487791/
Astaxanthin Inhibits PC-3 Xenograft Prostate Tumor Growth in Nude Mice
Xiaofeng Ni,1 Haining Yu,2 Shanshan Wang,1 Chengcheng Zhang,1 and Shengrong Shen1,* Keith B. Glaser, Academic Editor
Abstract
Prostate cancer (PCa), the most common malignancy in men, is a major cause of cancer deaths. A better understanding of the mechanisms that drive tumor initiation and progression may identify actionable targets to improve treatment of this patient group. As a dietary carotenoid, astaxanthin has been demonstrated to exert beneficial effects against inflammation, cardiovascular disease, oxidative damage, or different cancer sites. This study used intragastric administration of astaxanthin to detect its role on tumor proliferation, apoptosis, microRNA (miRNA) overexpression, and microbacteria composition change by establishing androgen-independent PCa cell PC-3 xenograft nude mice. Nude mice were inoculated with androgen-independent prostate cancer PC-3 cells subcutaneously. The intervention was started when tumors reached 0.5–0.6 cm in diameter. Mice were intragastrically administered 100 mg/kg astaxanthin (HA), 25 mg/kg astaxanthin (LA), or olive oil (TC). The results showed that 100 mg/kg astaxanthin significantly inhibited tumor growth compared to the TC group, with an inhibitory rate of 41.7%. A decrease of Ki67 and proliferating cell nuclear antigen (PCNA) as well as an increase of cleaved caspase-3 were observed in HA-treated tumors, along with increasing apoptotic cells, obtained by TUNEL assay. The HA significantly elevated the levels of tumor suppressors miR-375 and miR-487b in tumor tissues and the amount of Lactobacillus sp. and Lachnospiraceae in mice stools, while there was no significant difference between LA and TC groups. These results provide a promising regimen to enhance the therapeutic effect in a dietary supplement manner.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5367023/
J Herb Pharmacother. 2005;5(1):17-26.
A preliminary investigation of the enzymatic inhibition of 5alpha-reduction and growth of prostatic carcinoma cell line LNCap-FGC by natural astaxanthin and Saw Palmetto lipid extract in vitro.
Anderson ML1.
Abstract
Inhibition of 5alpha-reductase has been reported to decrease the symptoms of benign prostate hyperplasia (BPH) and possibly inhibit or help treat prostate cancer. Saw Palmetto berry lipid extract (SPLE) is reported to inhibit 5alpha-reductase and decrease the clinical symptoms of BPH. Epidemiologic studies report that carotenoids such as lycopene may inhibit prostate cancer. In this investigation the effect of the carotenoid astaxanthin, and SPLE were examined for their effect on 5alpha-reductase inhibition as well as the growth of prostatic carcinoma cells in vitro. These studies support patent #6,277,417 B1. The results show astaxanthin demonstrated 98% inhibition of 5alpha-reductase at 300 microg/mL in vitro. Alphastat, the combination of astaxanthin and SPLE, showed a 20% greater inhibition of 5alpha-reductase than SPLE alone n vitro. A nine day treatment of prostatic carcinoma cells with astaxanthin in vitro produced a 24% decrease in growth at 0.1 mcg/mL and a 38% decrease at 0.01 mcg/mL. SPLE showed a 34% decrease at 0.1 mcg/mL.
CONCLUSIONS:
Low levels of carotenoid astaxanthin inhibit 5alpha-reductase and decrease the growth of human prostatic cancer cells in vitro. Astaxanthin added to SPLE shows greater inhibition of 5alpha-reductase than SPLE alone in vitro.
